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Half Fraser (Demi-Fraser) Broth, 500 g
£
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Half Fraser (Demi-Fraser) Broth is used with ferric ammonium citrate for the selective enrichment of Listeria species and is not intended for use in the diagnosis of disease or other conditions in humans.
Primary enrichment broth for the isolation of Listeria spp. formulated according to ISO 11290. The selective components acriflavine and nalidixic acid are blended into the base powder and the ferric ammonium citrate is added to the tempered broth after sterilization.
Half Fraser (Demi-Fraser) Broth, NCM0001 was developed to give improved results for the isolation of Listeria spp. with both traditional and ELISA methods. As with the sister product, Fraser Broth, NCM0050 the selective components acriflavine and nalidixic acid are blended into the base powder and the Ferric Ammonium Citrate is added to the tempered broth after autoclaving. This format has been shown to give improved selectivity with pure cultures and food samples. Furthermore, more stable ELISA results are seen, resulting in fewer false positive results when the ferric ammonium citrate is omitted from the complete media.
Developed as a modification of UVM medium (NCM0012) and made according to ISO 11290, Fraser Broth is a secondary enrichment broth for the isolation of Listeria spp. and is similar to Palcam Broth (NCM0049) in that it contains esculin to indicate the presence of a potential Listeria isolate. It also contains lithium chloride in an attempt to suppress the growth of Enterococci in the medium (as does Palcam). Listeria spp. hydrolyze the esculin to form esculetin, which reacts with the ferric ammonium citrate in resulting in a black precipitate and a visible positive reaction. However, Enterococci can also perform this reaction so further plating is required onto an isolation medium such as Harlequin® Listeria Chromogenic Agar (NCM1004).
Half Fraser (Demi-Fraser) Broth, 5 kg
£
-
Half Fraser (Demi-Fraser) Broth is used with ferric ammonium citrate for the selective enrichment of Listeria species and is not intended for use in the diagnosis of disease or other conditions in humans.
Primary enrichment broth for the isolation of Listeria spp. formulated according to ISO 11290. The selective components acriflavine and nalidixic acid are blended into the base powder and the ferric ammonium citrate is added to the tempered broth after sterilization.
Half Fraser (Demi-Fraser) Broth, NCM0001 was developed to give improved results for the isolation of Listeria spp. with both traditional and ELISA methods. As with the sister product, Fraser Broth, NCM0050 the selective components acriflavine and nalidixic acid are blended into the base powder and the Ferric Ammonium Citrate is added to the tempered broth after autoclaving. This format has been shown to give improved selectivity with pure cultures and food samples. Furthermore, more stable ELISA results are seen, resulting in fewer false positive results when the ferric ammonium citrate is omitted from the complete media.
Developed as a modification of UVM medium (NCM0012) and made according to ISO 11290, Fraser Broth is a secondary enrichment broth for the isolation of Listeria spp. and is similar to Palcam Broth (NCM0049) in that it contains esculin to indicate the presence of a potential Listeria isolate. It also contains lithium chloride in an attempt to suppress the growth of Enterococci in the medium (as does Palcam). Listeria spp. hydrolyze the esculin to form esculetin, which reacts with the ferric ammonium citrate in resulting in a black precipitate and a visible positive reaction. However, Enterococci can also perform this reaction so further plating is required onto an isolation medium such as Harlequin® Listeria Chromogenic Agar (NCM1004).
Half Fraser (Demi-Fraser) Broth, 10 kg
£
-
Half Fraser (Demi-Fraser) Broth is used with ferric ammonium citrate for the selective enrichment of Listeria species and is not intended for use in the diagnosis of disease or other conditions in humans.
Primary enrichment broth for the isolation of Listeria spp. formulated according to ISO 11290. The selective components acriflavine and nalidixic acid are blended into the base powder and the ferric ammonium citrate is added to the tempered broth after sterilization.
Half Fraser (Demi-Fraser) Broth, NCM0001 was developed to give improved results for the isolation of Listeria spp. with both traditional and ELISA methods. As with the sister product, Fraser Broth, NCM0050 the selective components acriflavine and nalidixic acid are blended into the base powder and the Ferric Ammonium Citrate is added to the tempered broth after autoclaving. This format has been shown to give improved selectivity with pure cultures and food samples. Furthermore, more stable ELISA results are seen, resulting in fewer false positive results when the ferric ammonium citrate is omitted from the complete media.
Developed as a modification of UVM medium (NCM0012) and made according to ISO 11290, Fraser Broth is a secondary enrichment broth for the isolation of Listeria spp. and is similar to Palcam Broth (NCM0049) in that it contains esculin to indicate the presence of a potential Listeria isolate. It also contains lithium chloride in an attempt to suppress the growth of Enterococci in the medium (as does Palcam). Listeria spp. hydrolyze the esculin to form esculetin, which reacts with the ferric ammonium citrate in resulting in a black precipitate and a visible positive reaction. However, Enterococci can also perform this reaction so further plating is required onto an isolation medium such as Harlequin® Listeria Chromogenic Agar (NCM1004).
Half Fraser (Demi-Fraser) Broth, 25 kg (European only size)
£
-
Half Fraser (Demi-Fraser) Broth is used with ferric ammonium citrate for the selective enrichment of Listeria species and is not intended for use in the diagnosis of disease or other conditions in humans.
Primary enrichment broth for the isolation of Listeria spp. formulated according to ISO 11290. The selective components acriflavine and nalidixic acid are blended into the base powder and the ferric ammonium citrate is added to the tempered broth after sterilization.
Half Fraser (Demi-Fraser) Broth, NCM0001 was developed to give improved results for the isolation of Listeria spp. with both traditional and ELISA methods. As with the sister product, Fraser Broth, NCM0050 the selective components acriflavine and nalidixic acid are blended into the base powder and the Ferric Ammonium Citrate is added to the tempered broth after autoclaving. This format has been shown to give improved selectivity with pure cultures and food samples. Furthermore, more stable ELISA results are seen, resulting in fewer false positive results when the ferric ammonium citrate is omitted from the complete media.
Developed as a modification of UVM medium (NCM0012) and made according to ISO 11290, Fraser Broth is a secondary enrichment broth for the isolation of Listeria spp. and is similar to Palcam Broth (NCM0049) in that it contains esculin to indicate the presence of a potential Listeria isolate. It also contains lithium chloride in an attempt to suppress the growth of Enterococci in the medium (as does Palcam). Listeria spp. hydrolyze the esculin to form esculetin, which reacts with the ferric ammonium citrate in resulting in a black precipitate and a visible positive reaction. However, Enterococci can also perform this reaction so further plating is required onto an isolation medium such as Harlequin® Listeria Chromogenic Agar (NCM1004).
Half Fraser (Demi-Fraser) Broth, 50 kg (US only size)
£
-
Half Fraser (Demi-Fraser) Broth is used with ferric ammonium citrate for the selective enrichment of Listeria species and is not intended for use in the diagnosis of disease or other conditions in humans.
Primary enrichment broth for the isolation of Listeria spp. formulated according to ISO 11290. The selective components acriflavine and nalidixic acid are blended into the base powder and the ferric ammonium citrate is added to the tempered broth after sterilization.
Half Fraser (Demi-Fraser) Broth, NCM0001 was developed to give improved results for the isolation of Listeria spp. with both traditional and ELISA methods. As with the sister product, Fraser Broth, NCM0050 the selective components acriflavine and nalidixic acid are blended into the base powder and the Ferric Ammonium Citrate is added to the tempered broth after autoclaving. This format has been shown to give improved selectivity with pure cultures and food samples. Furthermore, more stable ELISA results are seen, resulting in fewer false positive results when the ferric ammonium citrate is omitted from the complete media.
Developed as a modification of UVM medium (NCM0012) and made according to ISO 11290, Fraser Broth is a secondary enrichment broth for the isolation of Listeria spp. and is similar to Palcam Broth (NCM0049) in that it contains esculin to indicate the presence of a potential Listeria isolate. It also contains lithium chloride in an attempt to suppress the growth of Enterococci in the medium (as does Palcam). Listeria spp. hydrolyze the esculin to form esculetin, which reacts with the ferric ammonium citrate in resulting in a black precipitate and a visible positive reaction. However, Enterococci can also perform this reaction so further plating is required onto an isolation medium such as Harlequin® Listeria Chromogenic Agar (NCM1004).
Buffered Peptone Water (BPW), 500 g
£
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Buffered Peptone Water (BPW) is used for the non-selective pre-enrichment of Salmonella spp. from food and is not intended for use in the diagnosis of disease or other conditions in humans.
Edel and Kamelmacher found that food preservation techniques involving heat, desiccation, preservatives, high osmotic pressure, or pH changes cause sublethal injury to Salmonella spp. Preenrichment in a non-selective medium allows for repair of cell damage and facilitates the recovery of Salmonella. Lactose Broth is frequently used for this purpose, but it may be detrimental to recovering Salmonellae. Buffered Peptone Water maintains a high pH over the pre-enrichment period and allows in repair of injured cells that may be sensitive to low pH. This is particularly important for vegetable specimens which have a low buffering capacity. Buffered Peptone Water is used in standard methods.
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