Buffered Peptone Water (BPW) is used for the non-selective pre-enrichment of Salmonella spp. from food and is not intended for use in the diagnosis of disease or other conditions in humans.
Edel and Kamelmacher found that food preservation techniques involving heat, desiccation, preservatives, high osmotic pressure, or pH changes cause sublethal injury to Salmonella spp. Preenrichment in a non-selective medium allows for repair of cell damage and facilitates the recovery of Salmonella. Lactose Broth is frequently used for this purpose, but it may be detrimental to recovering Salmonellae. Buffered Peptone Water maintains a high pH over the pre-enrichment period and allows in repair of injured cells that may be sensitive to low pH. This is particularly important for vegetable specimens which have a low buffering capacity. Buffered Peptone Water is used in standard methods.
| Formula | Liter |
| Enzymatic Digest of Casein | 10.0 g/L |
| Sodium Chloride | 5.0 g/L |
| Disodium Hydrogen Phosphate (anhydrous) | 3.5 g/L |
| Potassium Dihydrogen Phosphate | 1.5 g/L |
Final pH: 7.2 ± 0.2 at 25°C
Formula may be adjusted and/or supplemented as required to meet performance specifications.
Preparation
- Dissolve 20 g of the medium in one liter of purified water.
- Mix thoroughly.
- Autoclave at 121°C for 15 minutes.
Product was previously known as Acumedia Product: 7418 Buffered Peptone Water; and LabM Product: LAB046 Buffered Peptone Water
Order Details
| Product # | Product Description |
| NCM0003A | Buffered Peptone Water (BPW), 500 g |
| NCM0003B | Buffered Peptone Water (BPW), 5 kg |
| NCM0003C | Buffered Peptone Water (BPW), 10 kg |
| NCM0003D | Buffered Peptone Water (BPW), 25 kg (European only size) |
| NCM0003E | Buffered Peptone Water (BPW), 50 kg (US only size) |
Reference Material
NCM0003 - Buffered Peptone Water (BPW), Safety Data Sheet [Danish] [Dutch] [English] [EU English] [Finnish] [French] [German] [Italian] [Spanish, Mexico] [Swedish]
NCM0003 - Buffered Peptone Water (BPW), Technical Specifications Sheet [English]
For additional information or questions about product SDS documents, please contact Neogen at SDS@neogen.com.