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Microgen Salmonella latex confirmation assay
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Microgen Salmonella latex confirmation assay
Product Code: F42
A rapid test for the confirmatory identification of presumptive Salmonella colonies from selective agar plates. The genus Salmonella consists of over 2,000 serotypes which can cause acute infectious gastroenteritis frequently associated with food poisoning. Since many Salmonella spp. infect domestic animals, either clinically or sub-clinically, cases of Salmonella food poisoning usually originate from animal sources. The rapid identification of Salmonella is not only vital for appropriate patient management but also for food manufacturers where prompt results minimise the delay associated with routine testing for Salmonella contamination. The detection and identification of Salmonella spp., using traditional techniques, usually requires identification by serological and biochemical techniques. Microgen Salmonella offers a rapid alternative to the traditional identification methods and detects Salmonella spp. including S. typhimurium and S. enteritidis. Microgen Salmonella…. · Has a very high specificity and a high positive predictive value making it ideal for testing food, clinical, environmental and veterinary samples. · Detects motile and non-motile strains. · Produces test results in 2 minutes. · Obtained 100% sensitivity in a food sample trial that compared Microgen Salmonella with conventional identification techniques thus confirming highly reliable detection of Salmonella.
PLEASE NOTE THIS PRODUCT IS AVAILABLE FOR SALE IN THE UK ONLY.
Related Sector(s)
Clinical
Dairy
Food
Related Organism(s)
Salmonella
Microgen Listeria latex confirmation assay
£
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Microgen Listeria latex confirmation assay
Product Code: F48
A unique rapid test for the confirmatory identification of Listeria spp. from colonies on agar plate media. Listeria spp. are commonly found in soil and are transferred onto vegetable matter. Foods either derived from vegetables or which are derived from grazing animals and which are exposed to minimal processing i.e. dairy products, are the most common vector for the transmission of Listeria spp. to humans. A total of 6 species have been recognised to date including Listeria monocytogenes which has been associated with human disease. L. monocytogenes is an opportunistic pathogen in that it will only cause disease in people with certain predisposing factors such as the immunosuppressed and pregnant women.
Once colonies have been isolated on agar plates, the next stage requires that any colonies resembling Listeria spp. are tested to confirm that they belong to that genus. Other organisms may be isolated from food samples which may resemble Listeria spp. To confirm the presence of Listeria spp. a number of tests are normally used including aesculin hydrolysis, oxidase, catalase, motility (differential at 25°C and 37°C).
Microgen Listeria latex was developed as an alternative to this battery of tests. Although individually these tests are inexpensive, they are time consuming to perform. The use of a simple latex agglutination test provides a real-time 2 minute test for the confirmation to genus level of Listeria spp. Conventional culture methods for Listeria spp. rely on a combination of enrichment and selective media to achieve satisfactory growth of the organism These conventional methods are time consuming and result in unnecessary delays in releasing product from quarantine. Microgen Listeria can be used to obtain rapid, presumptive-positive results from selective media thus giving an early indication of a failure in the production process. Where standard methods require it, Microgen Listeria can be used to screen colonies before full biochemical identification and can replace traditional agglutinating antibody methods.
PLEASE NOTE THIS PRODUCT IS AVAILABLE FOR SALE IN THE UK ONLY.
Related Sector(s)
Clinical
Dairy
Food
Related Organism(s)
Listeria
Microscreen Clostridium difficile latex confirmation assay
£
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Microscreen Clostridium difficile latex confirmation assay
Product Code: M41
A unique rapid test for the confirmatory identification of Clostridium difficile cultured on selective solid media from faecal samples.
Clostridium difficile has a well documented role in pseudomembraneous colitis and diarrhoea associated with antibiotics. Evidence has also implicated C. difficile in colitis not induced by antibiotics, exacerbations of chronic inflammatory bowel disease and postoperative diarrhoea. Many laboratories rely on the detection of C. difficile cytotoxin to diagnose infection. However, investigations have shown that the detection of faecal cytotoxin is not always consistent with active infection. This, plus the fact that some laboratories do not have the specialised facilities that are required to detect faecal cytotoxin, has highlighted the need for a test that is easy to use and gives rapid results for the identification of C. difficile.
Use of Microgen® C. difficile allows identification of C. difficile from selective agar, within two minutes.
Traditional methods for identification of C. difficile includes GLC and cytotoxin detection. However, GLC is expensive in terms of time, equipment and cost per test and the link between presence of cytotoxin and symptoms is inconsistent. The test latex particles in Microgen® C. difficile are coated with antibodies specific for C. difficile somatic antigens, resulting in a highly specific test with a high predictive negative value.
The flexibility of the test means that it can be used to test isolates directly from selective agar plates, such as CCFA. The kit, which is simple-to-use and gives clear cut results, contains a positive control, has a long shelf-life, and offers the confidence of high sensitivity and specificity.
50 tests per pack
PLEASE NOTE THIS PRODUCT IS AVAILABLE FOR SALE IN THE UK ONLY.
Related Sector(s)
Clinical
Dairy
Food
Related Organism(s)
Clostridia
Microscreen Staph latex confirmation assay
£
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Microscreen Staph latex confirmation assay
Product Code: M43
A rapid test for the confirmatory identification of presumptive Staphylococcus aureus colonies from primary plate culture.
Two of the traditional diagnostic tests for S. aureus, the tube and slide coagulase tests, only detect the activity of a characteristic coagulase enzyme. However, these tests can be difficult to interpret and are liable to suffer from false positive results associated with non-specific factors frequently found in plasma. Non-specific coagulation due to other bacterial enzymes may also occur. However, 99% of S. aureus isolates also produce detectable levels of protein A on their surface, a characteristic which can be used to differentiate S. aureus from other species of Staphylococci in which this protein is rare.
Microgen® Staph is a test for the identification of S. aureus which overcomes the problems associated with the traditional tests by detecting both coagulase and protein A offering rapid and accurate identification of S. aureus in 2 minutes. Microgen® Staph contains all ancillary reagents needed to complete the test and is suitable for clinical, food and environmental laboratories.
Staphylococcus spp. occur as commensal organisms on the mucous membranes and skin of humans. However, Staphylococcus aureus can be responsible for a wide range of pyogenic infections including superficial suppurant lesions, food poisoning and toxic shock, as well as a variety of other conditions. It is therefore one of the most important pathogens regularly encountered in the laboratory.
Due to the frequency and importance of Staphylococcal infection a rapid and accurate diagnosis is needed for correct patient management, especially as the bacterium can show resistance to numerous common antibiotics. Food manufacturers find Staphylococcal detection to be a good indicator of the general level of microbial contamination. In these circumstances a highly specific test is needed to identify S. aureus, often from heavily contaminated samples. Traditional identification methods, and a number of commercially available products, may suffer from false positive results due to non-specific reactions, and false negative results due to an inability to detect certain strains of S. aureus.
Microgen® Staph is a sensitive and specific test for the identification of S. aureus from primary plate culture.
50 tests per pack
Related Sector(s)
Clinical
Dairy
Food
Related Organism(s)
Staphylococci
Microscreen Staph latex confirmation assay
£
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Microscreen Staph latex confirmation assay
Product Code: M43
A rapid test for the confirmatory identification of presumptive Staphylococcus aureus colonies from primary plate culture.
Two of the traditional diagnostic tests for S. aureus, the tube and slide coagulase tests, only detect the activity of a characteristic coagulase enzyme. However, these tests can be difficult to interpret and are liable to suffer from false positive results associated with non-specific factors frequently found in plasma. Non-specific coagulation due to other bacterial enzymes may also occur. However, 99% of S. aureus isolates also produce detectable levels of protein A on their surface, a characteristic which can be used to differentiate S. aureus from other species of Staphylococci in which this protein is rare.
Microgen® Staph is a test for the identification of S. aureus which overcomes the problems associated with the traditional tests by detecting both coagulase and protein A offering rapid and accurate identification of S. aureus in 2 minutes. Microgen® Staph contains all ancillary reagents needed to complete the test and is suitable for clinical, food and environmental laboratories.
Staphylococcus spp. occur as commensal organisms on the mucous membranes and skin of humans. However, Staphylococcus aureus can be responsible for a wide range of pyogenic infections including superficial suppurant lesions, food poisoning and toxic shock, as well as a variety of other conditions. It is therefore one of the most important pathogens regularly encountered in the laboratory.
Due to the frequency and importance of Staphylococcal infection a rapid and accurate diagnosis is needed for correct patient management, especially as the bacterium can show resistance to numerous common antibiotics. Food manufacturers find Staphylococcal detection to be a good indicator of the general level of microbial contamination. In these circumstances a highly specific test is needed to identify S. aureus, often from heavily contaminated samples. Traditional identification methods, and a number of commercially available products, may suffer from false positive results due to non-specific reactions, and false negative results due to an inability to detect certain strains of S. aureus.
Microgen® Staph is a sensitive and specific test for the identification of S. aureus from primary plate culture.
50 tests per pack
Related Sector(s)
Clinical
Dairy
Food
Related Organism(s)
Staphylococci
Microscreen E. coli O157 latex confirmation assay
£
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Microscreen E. coli O157 latex confirmation assay
Product Code: M44
A rapid test for the confirmatory identification of E.coli serogroup O157 cultured on selective solid media. Much of the rapidly expanding interest in E. coli O157 results from the increasing capability to isolate and identify this organism. E. coli O157 is a verotoxin (VT) producing strain responsible for Haemorrhagic colitis (HC), Haemolytic Uraemic Syndrome (HUS) and community acquired diarrhoea. Until recently it was thought that E. coli O157 was only isolated from samples of bloody diarrhoea as this is classically seen with verotoxin producing strains.
However, it is now increasingly being isolated from non-bloody specimens. Sorbitol MacConkey medium is the recommended screening agar for E. coli O157, but, although this technique shows 100% sensitivity it is only 85% specific. E. coli O157 does not ferment sorbitol. However, other sorbitol negative Gram negative rods also grow on sorbitol MacConkey agar. As a result of this, a specific rapid identification method is required. Microgen E. coli O157 is an easy-to-use one step, latex identification kit which gives results in under two minutes and specifically identifies E. coli O157 directly from the selective plate, even in mixed culture. The conventional method for identification of E. coli O157:H7 utilises specific antisera in a standard agglutination test.
However, this method and other commercially available latex tests have been shown to suffer from cross reactions with other enteric organisms such as E. hermanii, which antigenically and biochemically resembles E. coli O157, resulting in false positive results. Microgen E. coli O157 contains a test latex which has been sensitised with E. coli O157 specific antibodies, that are prepared by absorption with potentially cross reacting strains. Microgen E. coli O157 has demonstrated no cross reactions when tested against a panel of 161 E. coli O157 ‘O’ serotypes and 56 ‘H’ serotypes, and shows strong specific agglutination. Microgen E. coli O157 gives strong and specific results within two minutes and comes complete with a control latex which can be easily compared with test results to confirm non-specific reactions.
PLEASE NOTE THIS PRODUCT IS AVAILABLE FOR SALE IN THE UK ONLY.
Related Sector(s)
Clinical
Dairy
Food
Related Organism(s)
E.coli O157
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