RNase-Fee Water
£
RNase-Fee Water
Description
DEPC-treated to eliminate enzyme activity and then autoclaved, this sterile highly purified water product is perfect for use in PCR and Northern blotting techniques. RNase-Free water is available either as a single 250ml bottle or in fifty 5ml aliquots to prevent cross-contamination.
Catalogue Page Number: 28
| SKU | RFW250 | RFW50X5 |
|---|
This product has been cited in the following research papers:
| Reference title | Date | Citation | Product usage |
|---|---|---|---|
| Quantitative assessment of individual populations within polymicrobial biofilms | 2018 | Susana Patrícia Lopes, Nuno Filipe Azevedo & Maria Olívia Pereira, Scientific Reports 8, 9494 | …mixed by inverting the tubes and centrifuged for 23 min (12000 × g). Lastly, the supernatant was discarded and the pellet was allowed to air-dry, before resuspended it in 30 μL with RNAse free water (Cleaver Scientific Ltd, Warwickshire, UK). The concentration and purity of the total gDNA was spectrometrically assessed using a NanoDrop 1000™ (Thermo Scientific, Waltham, MA, US). The absorbance rat… |
| Glycine receptor subunits expression in the developing rat retina | 2017 | Gustavo Sánchez-Chávez, Miguel Ángel Velázquez-Flores, Ruth Ruiz Esparza-Garrido, Rocío Salceda, Neurochemistry International, Volume 108, September 2017, Pages 177-182, | …g at 12,000 × g for 10 min. The resulting pellet was washed three times with 75% ethanol and the ethanol residues were removed by evaporation. Purified RNA was suspended in 50 μl nuclease-free water (cleaver scientific). Concentration and purity of RNA were evaluated in the NanoDrop Spectrophotometer (Thermo Scientific).… |
| Characterization of Staphylococcus epidermidis phage vB_SepS_SEP9 – a unique member of the Siphoviridae family | 2014 | Luís D. R. Melo, Sanna Sillankorva, Hans-Wolfgang Ackermann, Andrew M. Kropinski, Nuno Cerca, Research in Microbiology, Volume 165, Issue 8, October 2014, Pages 679-685, | … previous step, an equal volume of chloroform was atded. The supernatant was precipitated on ice with isopropanol 3 M sodium acetate (pH 4.6), air-dried and it was resuspended in nuclease-free water (cleaver scientific, Rugby, UK). Genome sequencing was performed on a 454 sequencing platform (Plate-forme d’Analyses Génomiques at Laval University, Québec, Canada) to 50-fold coverage. Sequence data … |
| Effect of progesterone on Candida albicans vaginal pathogenicity | 2014 | Carlos Tiago Alves, Sónia Silva, Leonel Pereira, David W. Williams, Mariana Henriques, International Journal of Medical Microbiology, Volume 304, Issue 8, November 2014, Pages 1011-1017, | … gene. Each reaction mixture consisted of 10 μL (working concentration) of SsoFast EvaGreen Supermix (Bio-Rad, Berkeley, USA), 0.2 μL of forward and reverse primers (50 μM) (Table 1), 5.6 μL of dH2O (cleaver scientific Ltd, UK), 4 μL of two-fold diluted cDNA samples together with the respective target gene primers. Negative controls (dH2O), as well as, non- reverse transcriptase controls (NRT) wer… |
| Effect of progesterone on Candida albicans vaginal pathogenicity | 2014 | Carlos Tiago Alves, Sónia Silva, Leonel Pereira, David W. Williams, Mariana Henriques, International Journal of Medical Microbiology, Volume 304, Issue 8, November 2014, Pages 1011-1017, | …), 0.2 μL of each primer (50 μM) designed previously (Table 1)) and 4 μL of DNA, in a final reaction volume of 20 μL. Negative controls were performed using a reaction mixture with nuclease free H2O (cleaver scientific Ltd, UK) substituting for the template DNA. Template DNA for each positive control was obtained from FFPE tissues after the step of DNA extraction described above. PCR cycling condi… |
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