DNA Ladders
£
DNA Ladders
Description
Pre-made and containing loading dye for immediate use, Cleaver Scientific’s ready-to-use (RTU) DNA Markers are specially formulated to run accurately and produce sharp, well defined ladders. Available in six molecular weight ranges and composed of discrete marker fragments isolated from restriction-digested proprietary plasmids, each DNA marker will remain stable for up to 6 months at room temperature, 12 months if kept in the fridge at 4°C or 24 months at -20°C. All Cleaver Scientific RTU Markers contain high intensity reference bands and may be used to perform size comparisons with DNA molecules ranging from the smallest of PCR fragments to large, linearised cosmid vectors.
Product Includes: 500 µl vial
Catalogue Page Number: 27
| SKU | CSL-MDNA-100BP | CSL-MDNA-100BPH | CSL-MDNA-1K | CSL-MDNA-50BP | CSL-MDNA-BR | CSL-MDNA-HR |
|---|---|---|---|---|---|---|
| Concentration | 50μg/500μl vial | 54μg/500μl vial | 50μg/500μl vial | 56μg/500μl vial | 86μg/500μl vial | 52μg/500μl vial |
| Storage Conditions | 6 months at 25°C, 12 months at 4°C & 24 months at - 20°C | 6 months at 25°C, 12 months at 4°C & 24 months at - 20°C | 6 months at 25°C, 12 months at 4°C & 24 months at - 20°C | 6 months at 25°C, 12 months at 4°C & 24 months at - 20°C | 6 months at 25°C, 12 months at 4°C & 24 months at - 20°C | 6 months at 25°C, 12 months at 4°C & 24 months at - 20°C |
| Size Range | 100-1500bp | 100-3000bp | 250bp-10kb | 50-1500bp | 100bp-10kb | 250bp-25kb |
| Number of bands | 11 | 12 | 13 | 17 | 19 | 14 |
| Reference bands | 500, 1500bp | 500, 1500bp | 1Kb, 3kb | 200, 500bp | 500bp, 1.5 & 3kb | 1Kb, 3kb |
| Recommended loading volume | 5μl/well | 5μl/well | 5μl/well | 5μl/well | 5μl/well | 5μl/well |
| Tracking dyes | Orange G, Xylene Cyanol FF, Bromophenol Blue | Orange G, Xylene Cyanol FF, Bromophenol Blue | Orange G, Xylene Cyanol FF, Bromophenol Blue | Orange G, Xylene Cyanol FF, Bromophenol Blue | Orange G, Xylene Cyanol FF, Bromophenol Blue | Orange G, Xylene Cyanol FF, Bromophenol Blue |
This product has been cited in the following research papers:
| Reference title | Date | Citation | Product usage |
|---|---|---|---|
| High vegetative compatibility diversity of Cryphonectria parasitica infecting sweet chestnut (Castanea sativa) in Britain indicates multiple pathogen introductions | 2018 | Pérez‐Sierra, A., Romón‐Ochoa, P., Gorton, C., Lewis, A., Rees, H., Van Der Linde, S. and Webber, J., High vegetative compatibility diversity of Cryphonectria parasitica infecting sweet chestnut (Castanea sativa) in Britain indicates multiple pathogen introductions. Plant Pathology. | …The concentration of each product was quantified by Gel Doc XR+ band quantification software in comparison with a CSL-MDNA-1Kb Latder (Cleaver Scientific Ltd)… |
| Genetic association study of C5178A and G10398A mitochondrial DNA variants with type 2 diabetes in Bangladeshi population | 2018 | Saha, S.K., Akther, J., Huda, N., Yasmin, T., Alam, M.S., Hosen, M.I., Hasan, A.M. and Nabi, A.N., 2018. Genetic association study of C5178A and G10398A mitochondrial DNA variants with type 2 diabetes in Bangladeshi population. Meta Gene. | …ely restricted fragments (sizes, 173 bp and 106 bp) when C allele and lanes 6 and 15 contain unrestricted fragments of 279 bp when A allele were present. Lane 13 indicates a 100 base pair DNA Latder (Cleaver Scientific, UK). Lanes 6 and 13 in (A) and (B) represent 50 bp and 100 bp DNA latders, respectively. Sanger sequencing was performed to validate the C5178A variants present in amplicons. (C) r… |
| Antimicrobial activity of bacteria isolated from Red Sea marine invertebrates | 2018 | El Samak, M., Solyman, S.M. and Hanora, A., 2018. Antimicrobial activity of bacteria isolated from Red Sea marine invertebrates. Biotechnology Reports. | …er of 10 picomole), 1 µl of 100 ng/µl DNA and RNase-free water to final volume of 25 µl. PCR amplicons were analyzed by electrophoresis in 1.2 % (w/v) agarose gel with a molecular size marker 100 bp (Cleaver scientific, Ltd, UK).… |
| Assessment of CdS quantum dots effect on UV damage to DNA using a DNA/quantum dots structured electrochemical biosensor and DNA biosensing in solution | 2017 | Veronika Svitkova, Jana Blaskovicova, Monika Tekelova, Brigitta Margit Kallai, Jan Labuda, Sensors and Actuators B: Chemical, Volume 243, May 2017, Pages 435-444, | …d stored at 4 °C. TAE (Tris-acetate-EDTA) buffer and ethidium bromide were purchased from AppliChem, Germany. Agarose, 1 kb DNA latder used as the DNA length marker and loading dye were obtained from cleaver scientific Ltd, UK. Mica Grade V-1 Muscovite was purchased from SPI Supplies, USA. Poly-l-lysine hydrobromide (m.w. 30 000–70 000) was purchased from Sigma-Aldrich. Other chemicals, obtained f… |
| An electrochemical DNA-based biosensor to study the effects of CdTe quantum dots on UV-induced damage of DNA | 2015 | Hlavata, L., Striesova, I., Ignat, T. et al. Microchim Acta (2015) 182: 1715. https://doi.org/10.1007/s00604-015-1502-z | …TAE (Tris-acetate-EDTA) buffer and ethidium bromide were purchased from AppliChem Germany (www.applichem.com). Agarose, 1 kb DNA latder and Loading dye were obtained from Cleaver Scientific Ltd (www.cleaverscientific.com). 1 kb DNA latder was used as the DNA lenght marker. Other chemicals, obtained from Mikrochem, Slovakia (www.mikrochem.com) or Lachema, Brno (www.erbalachema.com/cz), were of anal… |
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