CASE is a selective chromogenic agar for the detection of Salmonella that can be used as part of the ISO 6579-1:2017 workflow. It is not intended for use in the diagnosis of disease or other conditions in humans.
CASE is a selective chromogenic agar for the detection of Salmonella. It utilizes a dual chromogenic system to differentiate between Salmonella and non-target organisms that grow on the agar. The first chromogen is a target for esterase activity present in Salmonella species. Utilization of this chromogen results in blue/green colonies. The second chromogen is a target for ß-glucosidase present in non-target organisms that are able to grow on the agar and also possess esterase activity. Utilization of the second chromogen masks the utilization of the first resulting in black colonies in non-Salmonella. All other organisms are either inhibited or grow colorless on the agar.
The media is able to detect non-motile Salmonella (S.pullorum and S.gallinarum) as well as monophasic variants (1,4,[5],12:i:-). The media is also able to detect serovars that present weak esterase activity (S.dublin), and lactose positive strains (S.arizonae).
| Formula | Liter |
| Growth Mix | 10.5 g/L |
| Selective Mix | 11.0 g/L |
| Buffer | 7.0 g/L |
| Opacity Agents | 7.5 g/L |
| Chromogenic Mix | 1.4 g/L |
| Agar | 12.5 g/L |
| Water | 100mL |
Final pH: 7.2 ± 0.2 at 25°C
Formula may be adjusted and/or supplemented as required to meet performance specifications.
Preparation
- For detection and enumeration and Serotyping of Salmonella - Refer to ISO 6579-1:2017 where CASE can be used as the second plate alongside XLD.
Product was previously known as LabM Product: PIN013 Pinnacle™ Chromogenic Agar for Salmonella Esterase (CASE)
Order Details
| Product # | Product Description |
| NCM3008-20 | Chromogenic Agar for Salmonella Esterase (CASE) (20 x 90mm plates) |
Product is only available in certain countries. Please contact your account manager if you require furthur details.
Reference Material
NCM3008 - Chromogenic Agar for Salmonella Esterase (CASE) (20 x 90mm plates), Safety Data Sheet [Danish] [Dutch] [English] [EU English] [Finnish] [French] [German] [Hungarian] [Italian] [Spanish, Mexico] [Swedish]
NCM3008 - Chromogenic Agar for Salmonella Esterase (CASE) (20 x 90mm plates), Technical Specifications Sheet [English]
For additional information or questions about product SDS documents, please contact Neogen at SDS@neogen.com.