Sabouraud Dextrose Broth is used for the cultivation of fungi from sterile and non-sterile products. Sabouraud Dextrose Broth conforms to Harmonized USP/EP/JP Requirements and is not intended for use in the diagnosis of disease or other conditions in humans.
A medium recommended by the Harmonized Pharmacopoeia for the enrichment of Candida albicans from non-sterile samples and fungal test strain cultivation. Conforms to USP/EP/JP performance specifications. The peptones and dextrose provide a nutritious base for luxuriant fungal growth and the acidic pH affords selectivity against bacteria. Due to the high carbohydrate content and low pH this medium is highly sensitive to overheating. According to the Harmonized Pharmacopoeia, Sabouraud Dextrose Broth is used as an enrichment broth, with subculture performed onto Sabouraud Dextrose Agar.
| Formula | Liter |
| Dextrose | 20.0 g/L |
| Enzymatic Digest of Animal Tissue | 5.0 g/L |
| Enzymatic Digest of Casein | 5.0 g/L |
Final pH: 5.6 ± 0.2 at 25°C
Formula may be adjusted and/or supplemented as required to meet performance specifications.
Preparation
- Dissolve 30 grams of the medium in one liter of purified water.
- Heat with frequent agitation to completely dissolve the medium, if necessary.
- Autoclave at 121°C for 15 minutes
Product was previously known as Acumedia Product: 7617 Sabouraud Dextrose Broth; and LabM Product: HP013 Sabouraud Dextrose Broth
Order Details
| Product # | Product Description |
| NCM0147A | Sabouraud Dextrose Broth, 500 g |
| NCM0147B | Sabouraud Dextrose Broth, 5 kg |
| NCM0147C | Sabouraud Dextrose Broth, 10 kg |
| NCM0147D | Sabouraud Dextrose Broth, 25 kg (European only size) |
| NCM0147E | Sabouraud Dextrose Broth, 50 kg (US only size) |
Reference Material
NCM0147 - Sabouraud Dextrose Broth, Safety Data Sheet [Danish] [Dutch] [English] [EU English] [Finnish] [French] [German] [Italian] [Spanish, Mexico] [Swedish]
NCM0147 - Sabouraud Dextrose Broth, Technical Specifications Sheet [English]
For additional information or questions about product SDS documents, please contact Neogen at SDS@neogen.com.