Reinforced Clostridial Medium is used for the cultivation of anaerobes from food. This medium conforms to Harmonized USP/EP/JP Requirements and is not intended for use in the diagnosis of disease or other conditions in humans.
Reinforced Clostridial Medium (RCM) is recommended by the Harmonized Pharmacopoeia for the selective enrichment of Clostridia from non-sterile samples and conforms to USP/EP/JP performance specifications. Peptone, beef and yeast extract provide a source of nitrogen, essential vitamins and amino acids. Starch aids the detoxification of harmful metabolites and glucose is a fermentable carbohydrate. Sodium chloride provides osmotic balance and sodium acetate acts as a buffer. L-Cysteine hydrochloride acts as a reducing agent to create an anaerobic environment and maintain a low Eh. This is aided by the low level of agar which reduces the oxygen permeability through the medium. According to the Harmonized Pharmacopoeia, Reinforced Medium for Clostridia is used as a selective enrichment broth, with subculture performed onto Columbia Agar.
| Formula | Liter |
| Beef Extract | 10.0 g/L |
| Peptone | 10.0 g/L |
| Yeast Extract | 3.0 g/L |
| Soluble Starch | 1.0 g/L |
| Glucose Monohydrate | 5.0 g/L |
| Cysteine Hydrochloride | 0.5 g/L |
| Sodium Chloride | 5.0 g/L |
| Sodium Acetate | 3.0 g/L |
| Agar | 0.5 g/L |
Final pH: 6.8 ± 0.2 at 25°C
Formula may be adjusted and/or supplemented as required to meet performance specifications.
Preparation
- Suspend 38 grams of powder in 1 liter of purified water.
- Heat with frequent agitation to completely dissolve the medium, if necessary.
- Autoclave at 121°C for 15 minutes.
Product was previously known as Acumedia Product: 7714 Reinforced Clostridial Medium; and LabM Product: HP011 Reinforced Medium for Clostridia (USP/EP/JP)
Order Details
| Product # | Product Description |
| NCM0102A | Reinforced Clostridial Medium, 500 g |
| NCM0102B | Reinforced Clostridial Medium, 5 kg |
| NCM0102C | Reinforced Clostridial Medium, 10 kg |
| NCM0102D | Reinforced Clostridial Medium, 25 kg (European only size) |
| NCM0102E | Reinforced Clostridial Medium, 50 kg (US only size) |
Reference Material
NCM0102 - Reinforced Clostridial Medium, Safety Data Sheet [Danish] [Dutch] [English] [EU English] [Finnish] [French] [German] [Italian] [Spanish, Mexico] [Swedish]
NCM0102 - Reinforced Clostridial Medium, Technical Specifications Sheet [English]
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