Rappaport Vassiliadis Salmonella Enrichment Broth (USP/EP/JP)

Product Code: HP007

A medium recommended by the Harmonised European Pharmacopoeia for the selective enrichment of Salmonella from non-sterile pharmaceutical samples.

Conforms to USP/EP/JP performance specification. Malachite green and magnesium chloride act as selective agents, combined with high osmotic pressure and low pH to effectively inhibit non-target competitive organisms. Soya peptone provides a source of nitrogen and potassium phosphate acts as a buffer. Some Salmonella (S. typhi and S. choleraesuis) are known to be sensitive to Malachite green and as such may fail to grow. If these organisms are suspected an alternative selective enrichment broth (e.g. LAB202 Mueller-Kauffmann Tetrathionate novobiocin Broth - MKTTn) should be used in parallel. This formulation is hygroscopic and will produce a slight exothermic reaction when mixed with water. According to the Harmonised European Pharmacopoeia, Rappaport Vassiliadis Salmonella Enrichment Broth is used as a selective enrichment broth, with subculture performed onto Xylose Lysine Deoxycholate (XLD) agar.

Related Organism(s)

• Salmonella