MacConkey Broth is used for the detection of coliform bacteria in milk and water. Conforms to Harmonized USP/EP/JP Requirements and is not intended for use in the diagnosis of disease or other conditions in humans.
A medium recommended by the Harmonized USP/EP/JP for the selective enrichment of Escherichia coli from non-sterile samples. Conforms to Harmonized USP/EP/JP performance specification. Gelatin peptone provides a source of nitrogen, while lactose is a fermentable carbohydrate. Ox bile acts a selective agent inhibiting most Gram-positive organisms and bromocresol purple acts as a pH indicator. A color change from purple to yellow indicates growth of a bile tolerant, lactose fermenting organism such as Escherichia coli. According to the Harmonized USP/EP/JP, MacConkey Broth is used as a selective enrichment broth, with subculture performed onto MacConkey Agar.
| Formula | Liter |
| Pancreatic Digest of Gelatin | 20.0 g/L |
| Lactose Monohydrate | 10.0 g/L |
| Dehydrated Ox Bile | 5.0 g/L |
| Bromocresol Purple | 0.01 g/L |
Final pH: 7.3 ± 0.2 at 25°C
Formula may be adjusted and/or supplemented as required to meet performance specifications.
Preparation
- Dissolve 35 grams of the medium in one liter of purified water.
- Mix thoroughly.
- Dispense into tubes containing Durham tubes.
- Autoclave at 121°C for 15 minutes.
Product was previously known as Acumedia Product: 7185 MacConkey Broth; and LabM Product: HP005 MacConkey Broth (USP/EP/JP)
Order Details
| Product # | Product Description |
| NCM0060A | MacConkey Broth, 500 g |
| NCM0060B | MacConkey Broth, 5 kg |
| NCM0060C | MacConkey Broth, 10 kg |
| NCM0060D | MacConkey Broth, 25 kg (European only size) |
| NCM0060E | MacConkey Broth, 50 kg (US only size) |
Reference Material
NCM0060 - MacConkey Broth, Safety Data Sheet [Danish] [Dutch] [English] [EU English] [Finnish] [French] [German] [Italian] [Spanish, Mexico] [Swedish]
NCM0060 - MacConkey Broth, Technical Specifications Sheet [English]
For additional information or questions about product SDS documents, please contact Neogen at SDS@neogen.com.