MacConkey Agar is used for the isolation and differentiation of Gram-negative enteric bacilli in a laboratory setting. Conforms to Harmonized USP/EP/JP Requirements and FDA/BAM. MacConkey Agar is not intended for use in the diagnosis of disease or other conditions in humans.
A medium recommended by the Harmonized USP/EP/JP for isolation and identification of Escherichia coli from non-sterile products. Conforms to USP/EP/JP performance specification. Gelatin serves as source of carbon and nitrogen. Lactose is a fermentable carbohydrate and sodium chloride maintains the osmotic balance. Bile salts and crystal violet act as selective agents inhibiting many Gram-positive bacteria. Escherichia coli can ferment lactose to produce acid which results in a pH drop. This is indicated by neutral red resulting in pink colonies. Enough acid production will cause the precipitation of bile salts resulting in a bile precipitate or halo around lactose fermenting bacteria. Non-lactose fermenting bacteria such as Salmonella spp. grow but remain colorless with no bile precipitate. According to the Harmonized USP/EP/JP, MacConkey Broth is used as a selective enrichment broth, with subculture performed onto MacConkey Agar.
| Formula | Liter |
| Enzymatic Digest of Gelatin | 17.0 g/L |
| Enzymatic Digest of Casein | 1.5 g/L |
| Enzymatic Digest of Animal Tissue | 1.5 g/L |
| Lactose | 10.0 g/L |
| Bile Salts Mixture | 1.5 g/L |
| Sodium Chloride | 5.0 g/L |
| Neutral Red | 0.03 g/L |
| Crystal Violet | 0.001 g/L |
| Agar | 13.5 g/L |
Final pH: 7.1 ± 0.2 at 25ºC
Formula may be adjusted and/or supplemented as required to meet performance specifications.
Preparation
- Suspend 50 g of the medium in one liter of purified water.
- Heat with frequent agitation and boil for one minute to completely dissolve the medium.
- Autoclave at 121ºC for 15 minutes.
- Cool to 45-50°C.
Product was previously known as Acumedia Product: 7102 MacConkey Agar; and LabM Product: HP006 MacConkey Agar (USP/EP/JP)
Order Details
| Product # | Product Description |
| NCM0017A | MacConkey Agar, 500 g |
| NCM0017B | MacConkey Agar, 5 kg |
| NCM0017C | MacConkey Agar, 10 kg |
| NCM0017D | MacConkey Agar, 25 kg (European only size) |
| NCM0017E | MacConkey Agar, 50 kg (US only size) |
Reference Material
NCM0017 - MacConkey Agar, Safety Data Sheet [Danish] [Dutch] [English] [EU English] [Finnish] [French] [German] [Hungarian] [Italian] [Spanish, Mexico] [Swedish]
NCM0017 - MacConkey Agar, Technical Specifications Sheet [English]
For additional information or questions about product SDS documents, please contact Neogen at SDS@neogen.com.