Buffered Sodium Chloride-Peptone Broth pH 7.0 is the diluent specified by the Harmonized Pharmacopoeia for the microbiological examination of non-sterile products andconforms to Harmonized USP/EP/JP Requirements in a laboratory setting. Buffered Sodium Chloride-Peptone Broth pH 7.0 is not intended for use in the diagnosis of disease or other conditions in humans.
Buffered Sodium Chloride-Peptone Broth pH 7.0 is recommended by the Harmonized Pharmacopoeia (USP/EP/JP) for the microbiological examination of non-sterile products. The low level of peptone lessens the physiological shock experienced by microorganism when suspended in a diluent. The dual phosphate components create a buffered environment and sodium chloride maintains the osmotic balance.
| Formula | Liter |
| Potassium Dihydrogen Phosphate | 3.6 g/L |
| Disodium Hydrogen Phosphate Dihydrate | 5.8 g/L (equivalent to 0.067 M Phosphate) |
| Sodium Chloride | 4.3 g/L |
| Enzymatic Digest of Casein | 1.0 g/L |
Final pH: 7.0 ± 0.2 at 25°C
Formula may be adjusted and/or supplemented as required to meet performance specifications.
Preparation
- Dissolve 14.7 g of the medium in one liter of purified water.
- Mix thoroughly.
- Autoclave at 121°C for 15 minutes.
Product was previously known as Acumedia Product: 7732 Buffered Sodium Chloride – Peptone Solution, pH 7.0; and LabM Product: HP017 Buffered Sodium Chloride-Peptone Solution pH 7.0 (USP/EP/JP)
Order Details
| Product # | Product Description |
| NCM0156A | Buffered Sodium Chloride-Peptone Broth pH 7.0, 500 g |
| NCM0156B | Buffered Sodium Chloride-Peptone Broth pH 7.0, 5 kg |
| NCM0156C | Buffered Sodium Chloride-Peptone Broth pH 7.0, 10 kg |
| NCM0156D | Buffered Sodium Chloride-Peptone Broth pH 7.0, 25 kg (European only size) |
| NCM0156E | Buffered Sodium Chloride-Peptone Broth pH 7.0, 50 kg (US only size) |
Reference Material
NCM0156 - Buffered Sodium Chloride-Peptone Broth, Safety Data Sheet [Danish] [Dutch] [English] [EU English] [Finnish] [French] [German] [Italian] [Spanish, Mexico] [Swedish]
NCM0156 - Buffered Sodium Chloride-Peptone Broth, Technical Specifications Sheet [English]
For additional information or questions about product SDS documents, please contact Neogen at SDS@neogen.com.