Reinforced Clostridial Agar can be used for the enumeration of anaerobes by pour plate, shake tube or membrane filtration methods, and is not intended for use in the diagnosis of disease or other conditions in humans.
This is a solidified version of Reinforced Clostridial Medium (NCM0102) and can be used for the enumeration of anaerobes by pour plate, shake tube or membrane filtration methods. When solidified in tubes or bottles with minimal head space it can be used for anaerobic culture without the need for anaerobic atmosphere.
| Formula | Liter |
| Yeast Extract | 3.0 g/L |
| Beef Extract | 10.0 g/L |
| Peptone | 10.0 g/L |
| Glucose | 5.0 g/L |
| Soluble Starch | 1.0 g/L |
| Sodium Chloride | 5.0 g/L |
| Sodium Acetate | 3.0 g/L |
| L-Cysteine Hydrochloride | 0.5 g/L |
| Agar | 12.0 g/L |
Final pH: 6.8 ± 0.2 at 25°C
Formula may be adjusted and/or supplemented as required to meet performance specifications.
Preparation
- Suspend 49.5 grams of the medium in one liter of purified water.
- Heat with frequent agitation and boil for one minute to completely dissolve the medium.
- Autoclave at 121°C for 15 minutes.
- Cool to 45-50°C.
Product was previously known as LabM Product: LAB023 Reinforced Clostridial Medium Agar
Order Details
| Product # | Product Description |
| NCM0224A | Reinforced Clostridial Agar, 500 g |
| NCM0224B | Reinforced Clostridial Agar, 5 kg |
| NCM0224C | Reinforced Clostridial Agar, 10 kg |
| NCM0224D | Reinforced Clostridial Agar, 25 kg (European only size) |
| NCM0224E | Reinforced Clostridial Agar, 50 kg (US only size) |
Reference Material
NCM0224 - Reinforced Clostridial Agar, Safety Data Sheet [Danish] [Dutch] [English] [EU English] [Finnish] [French] [German] [Italian] [Spanish, Mexico] [Swedish]
NCM0224 - Reinforced Clostridial Agar, Technical Specifications Sheet [English]
For additional information or questions about product SDS documents, please contact Neogen at SDS@neogen.com.