Perfringens Agar (OPSP) is used for the cultivation of anaerobic microorganisms and is not intended for use in the diagnosis of disease or other conditions in humans.
Oleandomycin, Polymixin, Sulphadiazine, Perfringens (OPSP) agar, has been used as a standard medium for Clostridium perfringens for many years. This medium was developed by Handford in 1974 to overcome some of the problems associated with enumerating Clostridium perfringens in foods. The medium is buffered and utilizes sodium metabisulphite and liver extract as sources of H2S with ferric ammonium citrate as the indicator.
The medium is made selective with the addition of X109 Sulphadiazine and X110 Oleandomycin / Polymyxin supplements. Some strains of C. perfringens may demonstrate sensitivity to the sulphadiazine antibiotic (X109) in such cases use of NCM0077, TSC Perfringens Agar Base (with X194 D-Cycloserine) should be considered.
| Formula | Liter |
| Tryptone | 15.0 g/L |
| Yeast Extract | 5.0 g/L |
| Soy Peptone | 5.0 g/L |
| Liver Extract | 7.0 g/L |
| Ferric Ammonium Citrate | 1.0 g/L |
| Sodium Metabisulphite | 1.0 g/L |
| Tris Buffer | 1.5 g/L |
| Agar | 10.0 g/L |
Final pH: 7.3 ± 0.2 at 25°C
Formula may be adjusted and/or supplemented as required to meet performance specifications.
Preparation
- Suspend 45.5 grams of the medium and add to 1 liter of purified water.
- Heat with frequent agitation and boil for one minute to completely dissolve the medium.
- Autoclave at 121°C for 15 minutes.
- Allow to cool to 45-50°C before adding 2 vials each of selective supplements X109 and X110.
Product was previously known as LabM Product: LAB109 Perfringens Agar (OPSP)
Order Details
| Product # | Product Description |
| NCM0268A | Perfringens Agar (OPSP), 500 g |
| NCM0268B | Perfringens Agar (OPSP), 5 kg |
| NCM0268C | Perfringens Agar (OPSP), 10 kg |
| NCM0268D | Perfringens Agar (OPSP), 25 kg (European only size) |
| NCM0268E | Perfringens Agar (OPSP), 50 kg (US only size) |
Reference Material
NCM0268 - Perfringens Agar (OPSP), Safety Data Sheet [Danish] [Dutch] [English] [EU English] [Finnish] [French] [German] [Italian] [Spanish, Mexico] [Swedish]
NCM0268 - Perfringens Agar (OPSP), Technical Specifications Sheet [US English]
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