Membrane Lactose Glucuronide Agar (mLGA) is a medium for the detection and identification of E. coli and coliforms from water samples and is not intended for use in the diagnosis of disease or other conditions in humans.
Traditionally, membrane Lauryl Sulphate Broth (mLSB) has been used as the standard media for isolating coliforms (including E. coli) from water potentially contaminated with sewage. Harlequin membrane Lactose Glucuronide Agar (mLGA) is a modification of mLSB aimed at reducing costs by reducing the number of filters used per test sample and aiding in the recovery and identification of coliforms and E. coli. The medium has been modified from the mLSB formulation by the incorporation of X-glucuronide (BCIG), sodium pyruvate and agar.
X-glucuronide is a chromogenic substrate which detects the β-glucuronidase enzyme - highly specific for E. coli* - and allows for the presumptive isolation of E. coli. Sodium pyruvate aids the recovery of chlorine stressed organisms and agar is incorporated to remove the need for absorbent pads.
This medium is recommended for the enumeration of coliform bacteria and E. coli by a single membrane filtration technique in The Microbiology of Drinking Water 2009 (previously Report 71).
*96-97% of E. coli strains positive. A notable exception is E. coli O157:H7.
| Formula | Liter |
| Peptone | 40.0 g/L |
| Yeast Extract | 6.0 g/L |
| Lactose | 30.0 g/L |
| Phenol Red | 0.2 g/L |
| Sodium Lauryl Sulphate | 1.0 g/L |
| Sodium Pyruvate | 0.5 g/L |
| X-Glucuronide (BCIG) | 0.2 g/L |
| Agar | 10.0 g/L |
Final pH: 7.4 ± 0.2 at 25°C
Formula may be adjusted and/or supplemented as required to meet performance specifications.
Preparation
- Suspend 88 grams of the medium in one liter of purified water.
- Heat with frequent agitation and boil for one minute to completely dissolve the medium.
- Autoclave at 115oC for 10 minutes.
- Cool to 45-50°C.
Product was previously known as LabM Product: HAL009 Harlequin mLGA
Order Details
| Product # | Product Description |
| NCM1009A | Harlequin membrane Lactose Glucuronide Agar (mLGA), 500 g |
| NCM1009B | Harlequin membrane Lactose Glucuronide Agar (mLGA), 5 kg |
| NCM1009C | Harlequin membrane Lactose Glucuronide Agar (mLGA), 10 kg |
| NCM1009D | Harlequin membrane Lactose Glucuronide Agar (mLGA), 25 kg (European only size) |
| NCM1009E | Harlequin membrane Lactose Glucuronide Agar (mLGA), 50 kg (US only size) |
Reference Material
NCM1009 - Harlequin membrane Lactose Glucuronide Agar (mLGA), Safety Data Sheet [Danish] [Dutch] [English] [EU English] [Finnish] [French] [German] [Italian] [Spanish, Mexico] [Swedish]
NCM1009 - Harlequin membrane Lactose Glucuronide Agar (mLGA), Technical Specifications Sheet [English]
For additional information or questions about product SDS documents, please contact Neogen at SDS@neogen.com.