The HI84533-60 is a 30 mL bottle of hydrogen peroxide reagent that is necessary for wine and fruit juice samples that contain high levels of SO₂. The reagent neutralizes SO₂ present and ensures an accurate determination of formol number.

The content of amino acids and other nitrogen compounds in wine or fruit juice is determined by an acid base titration to a fixed pH endpoint and expressed as total assimilable nitrogen. The determination of formol number is possible only after neutralization of the titratable acids present by a pH adjustment with HI84533-62 reagent, followed by the blockage of the amino groups by reaction with the HI84533-61 formol base reagent. The titration is then performed with the HI84533-50 titrant solution.

Replacement reagents required to operate the HI84533 include: