omniPAGE Mini Vertical Protein Electrophoresis System
£
omniPAGE Mini Vertical Protein Electrophoresis System
Description
Cleaver Scientific Mini Vertical systems are predominantly used for protein electrophoresis.
By combining both functionality and ease of use, the omniPAGE systems set the bench mark for simple, versatile vertical mini gel electrophoresis. Each omniPAGE electrophoresis system can accommodate up to 4 handcast gels (additional plates required) or 2 commercial precast gels to provide complete flexibility for individual research needs, while unique sliding clamp technology within the PAGE insert facilitates fast, intuitive leak-free casting.
With a trouble-free set up and consistent performance, the omniPAGE Mini Vertical systems are perfect for today’s laboratories, where the ability to generate reproducible results quickly is essential.
Catalogue Page Number: 34
This unit has a 12 month limited warranty, for more information, consult our terms and conditions.
| SKU | CVS10D | CVS10DSYS | CVS10DSYS-CU | CVS10PRE |
|---|---|---|---|---|
| Unit Dimensions (cm) | 19 x 13 x 15 | 19 x 13 x 15 | 19 x 13 x 15 | 19 x 13 x 15 |
| Weight (Kg) | 1.8 | 1.8 | 1.8 | 1.8 |
| Capacity | 1 - 4 Gels | 1 - 4 Gels | 1 - 4 Gels | 1 - 4 Gels |
| Maximum Sample Capacity | 80 | 80 | 80 | 80 |
| Base Buffer Volume | 250 | 250 | 250 | 250 |
| Typical Running Conditions | 1-2 hours at 90-225V | 1-2 hours at 90-225V | 1-2 hours at 90-225V | 1-2 hours at 90-225V |
| Gel Dimensions | 8 x 8.5 | 8 x 8.5 | 8 x 8.5 | 8 x 8.5 |
| Maximum Buffer Volume (ml) | 1200 | 1200 | 1200 | 1200 |
| Includes Casting Base | FALSE | TRUE | TRUE | FALSE |
| Blotting Insert (optional) | VS10BI | VS10BI | VS10BI | VS10BI |
| 2D Insert (optional) | VS10DCI | VS10DCI | VS10DCI | VS10DCI |
| Includes Additional Caster | FALSE | FALSE | TRUE | FALSE |
| Plate Dimensions | 10 x 10 x 0.2 | 10 x 10 x 0.2 | 10 x 10 x 0.2 | 10 x 10 x 0.2 |
This product has been cited in the following research papers:
| Reference title | Date | Citation | Product usage |
|---|---|---|---|
| Detection of specific immune response in sole (Solea senegalensis) against Photobacterium damselae subsp. piscicida antigens | 2018 | Medina, A., García-Millán, I., Martínez-Manazanares, E., Moriñigo, M.A. and Arijo, S., 2018. Detection of specific immune response in sole (Solea senegalensis) against Photobacterium damselae subsp. piscicida antigens. Fish & Shellfish Immunology. | … Isoelectrofocusing (IEF) Cell, (BioRad).The IPG strips were placed on top of 10% acrylamide gel subjected to immunoblot analysis. Second dimension separations were performed on an Omnipage platform (Cleaver Scientific). Proteins were separated according their molecular weight at 250 V. Proteins in the gels were fixed with 50% methanol/10% acetic acid. The gels were stained by silver stain (Proteo… |
| Cell laden alginate-keratin based composite microcapsules containing bioactive glass for tissue engineering applications | 2018 | Reakasame, S., Trapani, D., Detsch, R. and Boccaccini, A.R., 2018. Cell laden alginate-keratin based composite microcapsules containing bioactive glass for tissue engineering applications. Journal of Materials Science: Materials in Medicine, 29(12), p.185. | …]. Sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS–PAGE) was carried out to characterize the keratin extract solution by using the OmniPAGE TETRAD Package (Cleaver Scientific Ltd.). The resolving gels (10% acrylamide of about 1.5 mm thickness) were run at a constant voltage (120 V) and prepared according to the method described by Laemmli [… |
| Immunoprotection elicited in rabbit by a chimeric protein containing B-cell epitopes of Sphingomyelinases D from Loxosceles spp. Spiders | 2018 | Souza, N.A., Dias-Lopes, C., Matoso, Í.H.G., de Oliveira, C.F.B., Chávez-Olortegui, C.D., Minozzo, J.C. and Felicori, L.F., 2018. Immunoprotection elicited in rabbit by a chimeric protein containing B-cell epitopes of Sphingomyelinases D from Loxosceles spp. spiders. Vaccine. | …lecular mass, a 20% polyacrylamide SDS-PAGE was used. The samples (15 μg of each) were boiled with 5% β-mercapto-etanol and the electrophoresis was performed using the vertical system CVS10 OmniPage (Cleaver Scientific Ltd) at 150 V for 2 h. To visualize the protein bands, the gel was transferred to the Coomassie Blue staining solution and incubated at room temperature overnight. The excess of sta… |
| Purification and Characterization of Lipase Produced by Leuconostoc mesenteroides Subsp. mesenteroides ATCC 8293 Using an Aqueous Two-Phase System (ATPS) Composed of Triton X-100 and Maltitol | 2018 | Sukohidayat, N.H.E., Zarei, M., Baharin, B.S. and Manap, M.Y., 2018. Purification and Characterization of Lipase Produced by Leuconostoc mesenteroides Subsp. mesenteroides ATCC 8293 Using an Aqueous Two-Phase System (ATPS) Composed of Triton X-100 and Maltitol. Molecules, 23, p.1800. | …SDS-PAGE analysis was performed using an omniPAGE Mini CVS10D vertical electrophoresis unit (Cleaver Scientific, Warwickshire, UK). The electrophoresis was conducted on a 4% polyacrylamide stacking gel as well as a 10% polyacrylamide-resolving gel and run at 120 V in Tris-glycine running buffer. The gel was then stained with Coomassie Brilliant Blue R-250. For the destaining of the gel, a destaini… |
| Soy protein isolate/bioactive glass composite membranes: processing and properties | 2018 | Tansaz, S., Schulte, M., Kneser, U., Mohn, D., Stark, W., Roether, J.A., Cicha, I. and Boccaccini, A.R., 2018. Soy protein isolate/bioactive glass composite membranes: processing and properties. European Polymer Journal. | …Sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS–PAGE) was carried out for MP Biomedicals SPI solution by using the OmniPAGE TETRAD Package from Cleaver Scientific Ltd. The resolving gels (10% acrylamide of about 1.5 mm thickness) were run at a constant voltage (120 V) and prepared according to the method described by Laemmli [24]. Proteins were visualized by Coomassie Brilliant blue… |
| Vectors for Expression of Signal Peptide-Dependent Proteins in Baculovirus/Insect Cell Systems and Their Application to Expression and Purification of the High-Affinity Immunoglobulin Gamma Fc Receptor I in Complex with Its Gamma Chain | 2018 | Le, L.T.M., Nyengaard, J.R., Golas, M.M. et al. Mol Biotechnol (2018) 60: 31. https://doi.org/10.1007/s12033-017-0041-8 | …SDS–PAGE gels were prepared following the protocol provided by the manufacturer (OmniPAGE Mini, Cleaver Scientific Ltd, Rugby, UK) with 30% acrylamide/bisacrylamide solution (Sigma-Aldrich). In the resolving gel, the acrylamide/bisacrylamide concentration was 12 or 15% according to target protein sizes, and in the stacking gel, the concentration was 4%. Samples were mixed with SDS loading buffer a… |
| Metabolic and functional distinction of the Smicronyx sp. galls on Cuscuta campestris | 2018 | Zagorchev, L.I., Albanova, I.A., Tosheva, A.G. et al. Planta (2018). https://doi.org/10.1007/s00425-018-2926-6 | …ble proteins, was used for subsequent analyses. Protein concentration was measured with Pierce BCA protein assay kit according to instruction manual. A total of 15 µg protein was loaded onto wells of Cleaver Scientific Omnipage Mini electrophoretic system. Separation was on 12.5% T SDS PAGE according to the protocol of Laemmli (1970), followed by renaturation in 2.5% (v/v) Triton® X-100 for 20 min… |
| The effect of chitosan–PMAA–NPK nanofertilizer on Pisum sativum plants | 2018 | Khalifa, N.S. & Hasaneen, M.N. 3 Biotech (2018) 8: 193. https://doi.org/10.1007/s13205-018-1221-3 | … electrophoresis (i.e., SDS-PAGE). Preparation and staining of the gel were carried out as described by Laemmli (1970). Electrophoresis was performed at 100 V using Omni-PAGE Mini Wide vertical unit (Cleaver Scientific, UK). Major protein bands of pea plants separated by SDS-PAGE electrophoresis were identified according to Barac et al. (2010).The experiment was repeated three times for confirming… |
| H2O2 homeostasis in wild-type and ethylene-insensitive Never ripe tomato in response to salicylic acid treatment in normal photoperiod and in prolonged darkness | 2018 | Zoltán Takács, Péter Poór, Péter Borbély, Zalán Czékus, Irma Tari, Plant Physiology and Biochemistry, Volume 126, May 2018, Pages 74-85, | …The electrophoretic separation of SOD, APX and POD isoenzymes was performed with native polyacrylamide gel electrophoresis (PAGE) using an omniPAGE electrophoresis system (cleaver scientific Ltd., Rugby, Warwickshire, UK). Electrophoresis was performed at 4 °C for 1–3 h at a constant voltage of 120 V using 25 mM Tris and 192 mM glycine solution (pH 8.3) as a running buffer. For SOD and APX, 4% sta… |
| A universal, rapid, and inexpensive method for genomic DNA isolation from the whole blood of mammals and birds | 2017 | SAHIB A. AL-SHUHAIB, M.B. J Genet (2017) 96: 171. https://doi.org/10.1007/s12041-017-0750-6 | …or 10 min and chilled on ice for at least 5 min. Then, the PCR amplicons were separated in a mini vertical gel electrophoresis format, gel size (W × L) cm: 10 × 10, and gel thickness: 1 mm (OmniPAGE, Cleaver Scientific, Warwickshire, UK). Denatured PCR products were loaded in 8% (37.5 acrylamide / 1 bis) polyacrylamide gels, containing 7% glycerol, and 1 × TBE buffer. The gels were run under 200 V… |
| Internalization of (bis)phosphonate-modified cellulose nanocrystals by human osteoblast cells | 2017 | Gorgieva, S., Vivod, V., Maver, U. et al. Cellulose (2017) 24: 4235. https://doi.org/10.1007/s10570-017-1432-5 | …e control sample, being the RBITC dye solution, was prepared in parallel, using the same procedure as for the CNC-labelled dispersions. The analysis was performed on an OmniPAGE mini-SDS-PAGE system (Cleaver Scientific) with a Consort EV 215 power supply and using prepared polyacrylamide gels (Precise, Thermo scientific, Germany). In each of 12 wells, 10 µL of the sample was loaded, and a power su… |
| In vitro significance of SOCS-3 and SOCS-4 and potential mechanistic links to wound healing | 2017 | Yi Feng, Andrew J. Sanders, Liam D. Morgan, Sioned Owen, Fiona Ruge, Keith G. Harding[…] & Wen G. Jiang- Show fewer authors, Scientific Reports 7, 6715 | …Tris-glycine SDS-PAGE was undertaken using an OmniPAGE VS10DSYS vertical electrophoresis system (OmniPAGE, Cleaver Scientific Ltd., Rugby, UK) to separate proteins before wet transfer to a PVDF membrane (EMD Millipore Corporation, Billerica, MA, USA).… |
| Modulation by NADPH oxidase of the chronic cardiovascular and autonomic interaction between cyclosporine and NSAIDs in female rats | 2017 | Ahmed F. El-Yazbi, Karim S. Ibrahim, Hanan M. El-Gowelli, Nevine M. El-Deeb, Mahmoud M. El-Mas, European Journal of Pharmacology, Volume 806, 5 July 2017, Pages 96-104, | …Protein extracts were then used for SDS-PAGE and blotting as described previously (El-Yazbi et al., 2010) with an electrophoresis setup from cleaver scientific Ltd. (Warwickshire, UK). Upon conclusion of the electrophoresis run, the polyacrylamide gel was cut at the 70 KD marker. The upper portion was used for transfer, while the lower portion was stained overnight at room temperature for actin in… |
| Prolonged dark period modulates the oxidative burst and enzymatic antioxidant systems in the leaves of salicylic acid-treated tomato | 2017 | Péter Poór, Zoltán Takács, Krisztina Bela, Zalán Czékus, Irma Tari, Journal of Plant Physiology, Volume 213, June 2017, Pages 216-226, | …NADPH oxidase activity was analysed using omniPAGE electrophoresis system (cleaver scientific Ltd., Rugby, Warwickshire, UK) as described by Carter et al. (2007). 0.5 g of leaf samples were crushed to a fine powder in a mortar under liquid N2 and then soluble proteins were extracted by resuspending the powder in 1 mL of 50 mM sodium phosphate buffer (pH 6.8) containing 0.5% Triton X-100. The homog… |
| Ectoine and hydroxyectoine inhibit thermal-induced aggregation and increase thermostability of recombinant human interferon Alfa2b | 2017 | Faranak Salmannejad, Nastaran Nafissi-Varcheh, European Journal of Pharmaceutical Sciences, Volume 97, 15 January 2017, Pages 200-207, | …erformed using a separating gel containing 12.5% (w/v) acrylamide and 0.1% SDS, and a stacking gel containing 3% (w/v) acrylamide and 0.1% SDS in the Omni PAGE mini vertical gel electrophoresis unit (cleaver scientific, Warwickshire, UK)·Gels were run under non-reducing condition (sample buffer without β-mercaptoethanol) at 200 V at room temperature. Protein bands were visualized by coomassie stai… |
| The simultaneous delivery of paclitaxel and Herceptin® using solid lipid nanoparticles: In vitro evaluation | 2016 | Gülay Büyükköroğlu, Behiye Şenel, Seval Gezgin, Tan Dinh, Journal of Drug Delivery Science and Technology, Volume 35, October 2016, Pages 98-105, | …ure were used as positive control. A 12% polyacrylamide gel was prepared and the samples were applied onto the gel in volumes of 40 μL.The SDS–PAGE was performed on a vertical electrophoresis system (cleaver scientific, UK) at constant voltage of 150 V for about 3 h. The gel was stained with Coomassie blue and de-stained in distilled water overnight. Images were obtained using a Kodak Image Statio… |
| Enzymatically-boosted ionic liquid gas separation membranes using carbonic anhydrase of biomass origin | 2016 | András Bednár, Nándor Nemestóthy, Péter Bakonyi, László Fülöp, Katalin Bélafi-Bakó, Chemical Engineering Journal, Volume 303, 1 November 2016, Pages 621-626, | …To confirm the presence of CA, SDS-PAGE was performed on a cleaver scientific Ltd, Nano-PAC – 300 gel apparatus with 4% acrylamide stacking gel and 12.5% acrylamide running gel. The samples were treated with SDS and 2-mercaptoethanol before running. The proteins on the SDS-PAGE gels were stained with Coomassie Blue R-250 and visualised by a GelAnalyzer 2010a image analysis software.… |
| Cocoa polyphenols exhibit antioxidant, anti-inflammatory, anticancerogenic, and anti-necrotic activity in carbon tetrachloride-intoxicated mice | 2016 | Jasminka Giacometti, Damir Muhvić, Adriano Pavletić, Luka Ðudarić, Journal of Functional Foods, Volume 23, May 2016, Pages 177-187, | …PAGE sample buffer (Invitrogen, Carlsbad, CA, USA) and heated at 95 °C for 5 min. The electrophoretic separation was performed with precast 4–12% Bis–Tris gels in an OmniPAGE mini, a vertical system (cleaver scientific Ltd, Rugby, Warwickshire, UK) according to the manufacturer’s procedure. The gels were stained with colloidal Coomassie Blue G-250 stain and visualised by a VersaDoc Imaging System … |
| Quercetin glycosides and chlorogenic acid in highbush blueberry leaf decoction prevent cataractogenesis in vivo and in vitro: Investigation of the effect on calpains, antioxidant and metal chelating properties | 2016 | Anastasia-Varvara Ferlemi, Olga E. Makri, Penelope G. Mermigki, Fotini N. Lamari, Constantinos D. Georgakopoulos, Experimental Eye Research, Volume 145, April 2016, Pages 258-268, | …Electrophoresis was performed on an instrument of cleaver scientific Ltd (Warwickshire, Rugby, UK) according to the modified method of Laemmli et al. (Laemmli, 1970). Lens homogenate (30 μg WSF and 250 μg WIF per lane) were mixed with sample buffer (35% v/v glycerol, 18% v/v 2-mercaptoethanol, 230 mM Tris/HCl pH = 6.8, 10% w/v sodium dodecyl sulfate, 0.3% w/v bromophenol blue), boiled for 5 min at… |
| Aging of low and high amylose rice at elevated temperature: Mechanism and predictive modeling | 2016 | Nonthacha Thanathornvarakul, Jirarat Anuntagool, Kanitha Tananuwong, Journal of Cereal Science, Volume 70, July 2016, Pages 155-163, | …litres of the mixture were then loaded onto precast SDS-PAGE gel, having 10% acrylamide in separating gel and 4% acrylamide in stacking gel, and subjected to electrophoresis (OmniPAGE mini-CVS10DSYS, cleaver scientific, Warwickshire, UK). Perfect Protein™ markers, 10–225 kDa (Novagen®, Merck Millipore, Madison, WI, USA), were used as standard for protein molecular weight determination. Duplicate a… |
| Identification of biogenic amines-producing lactic acid bacteria isolated from spontaneous malolactic fermentation of chilean red wines | 2016 | Karem Henríquez-Aedo, Daniel Durán, Apolinaria Garcia, Martha B. Hengst, Mario Aranda, LWT – Food Science and Technology, Volume 68, May 2016, Pages 183-189, | …gestion for 3 h at 37 °C inside the thermocycler block. The resulting DNA fragments (patterns) were separated by gel electrophoresis on 12% v/v polyacrylamide gel in a omniPAGE mini vertical chamber (cleaver scientific, Warmickshire, UK) applying 80 V. RFLP patterns were visualized under UV light using 0.5 μg mL−1 of ethidium bromide with 20 bp latder (Thermo Scientific) as a DNA molecular weight … |
| Identification of Vibrio harveyi proteins involved in the specific immune response of Senegalese sole (Solea senegalensis, Kaup) | 2015 | A. Medina, J. M. Mancera, E. Martínez-Manzanares, M. A. Moriñigo, S. Arijo, Fish & Shellfish Immunology, Volume 47, Issue 1, November 2015, Pages 377-380, | …ng a Protean Isoelectrofocusing (IEF) Cell, (BioRad). IPG strips were embetded in 0.5% agarose (w/v) on top of 10% acrylamide gel. Second dimension separations were performed on an Omnipage platform (cleaver scientific) at 250 V. Proteins in the gels were fixed with 50% methanol and 10% acetic acid. The gels were silver stained (Proteo Silver Plus, Sigma) and scanned at 700 ppi (GS-800, BioRad).… |
| Alkaline Hydrolysis of Chromium Tanned Leather Scrap Fibers and Anaerobic Biodegradation of the Products | 2014 | Ferreira, M.J., Almeida, M.F., Pinho, S.C. et al. Waste Biomass Valor (2014) 5: 551. https://doi.org/10.1007/s12649-013-9252-9 | …ated, by polyacrylamide gel electrophoresis in sodium dodecyl sulfate (SDS-PAGE), using a Major Scientific NM-300 N power source (Saratoga, USA), an electrophoresis system omniPage Mini Vertical from Cleaver Scientific Ld. (Warwickshire, UK) and specific consumables and standards from National Diagnostics (Yorkshire, UK). The gels were prepared and used according consumables provider instructions … |
| Purification of papain from unclarified papaya juice using reversed phase expanded bed adsorption chromatography (RP-EBAC) | 2014 | Yuhai He, Syed Mohd Saufi bin Tuan Chik, Fui Chin Chong, Journal of Industrial and Engineering Chemistry, Volume 20, Issue 6, 25 November 2014, Pages 4293-4297, | …al protein in each sample fraction was quantified using the Bradford Assay. The collected fractions of papain were subjected to electrophoresis at 200 V on a CSL OmniPAGE Mini Electrophoresis System (cleaver scientific Ltd., Rugby, UK) with acrylamide of 15% (w/v) and 5% (w/v) for resolving gel and stacking gel respectively.… |
| l-Dopa decarboxylase (tdC) constitutes an emerging biomarker in predicting patients’ survival with stomach adenocarcinomas | 2013 | Florou, D., Papadopoulos, I.N., Fragoulis, E.G. et al. J Cancer Res Clin Oncol (2013) 139: 297. https://doi.org/10.1007/s00432-012-1326-8 | …Fifty micrograms of total protein was separated on a 10 % SDS-polyacrylamide gel electrophoresis (SDS-PAGE) apparatus (Cleaver Scientific, UK) (Laemmli 1970) at 150 V for 1.5 h at room temperature. Transferring of proteins onto nitrocellulose sheets (Scheicher and Schuell Bioscience, Germany) (Towbin et al. 1979) took place inside a semidry apparatus (BioRad, UK). Membrane blockage in 10 % BSA occ… |
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