SpotQuest – 2D Gel Analysis Software

£

SpotQuest – 2D Gel Analysis Software

Description



SpotQuest is software with basic functionality and easy to use 10-step workflow. It makes it easy for small laboratories and novice users to apply 2D gel electrophoresis as part of molecular biology or biochemistry research. It gives you a quick, simple, objective way to detect and measure changes in protein samples using 2D gel image analysis.

 

 


 

    • 3 minutes to fully analyse 1,000 proteins on each gel image with no need for tedious, subjective spot detection and matching.

 

 


 

    • A 10-step sequence takes you from opening your 2D gel images to generating a map of differentially expressed proteins without setting parameters.

 

 


 

    • A guided automatic workflow means new or occasional users can generate consistent and reliable results every time.

 

 


 

SpotQuest features

 

 

    • Simple download and activate with an e-licence

 

    • Analyses single stain and DIGE experiments

 

    • Supports .tiff, .img, .png, .gel, .mel images

 

    • Automatically performs a range of image quality checks and provides feedback

 

    • Image manipulation tools include rotate, flip, invert and crop

 

    • Ability to exclude areas of the gel image from the analysis

 

    • Allows a highly accurate correction of image distortion through the placement of alignment vectors

 

    • Alignment review step allows adjustments if needed

 

    • Automatic analysis of aligned image set

       

        • Spot detection

       

        • Background subtraction

       

        • Normalisation

       

        • 100% matching of all spots across the experiment

       

       

 

    • Filter out spots or areas of the gel image to be excluded from further analysis

 

    • Group gel images according to experiment structure and spot fold change and ANOVA p-value displayed in review results step.

 

    • Review of spots using 2D / 3D montage view and spot data table

 

    • Spot edits on a single gel image copied to all other gel images in the experiment

 

    • Expression profile plot with error bar showing 3 standard errors within groups

 

    • Option to export spot measurements as .csv file

 

    • Spots for picking highlighted on the image view to guide manual picking

 

    • Report shows an overview of spots selected for picking

 



 

Catalogue Page Number: 67

سجل معنا في القائمة البريدية ليصل اليك كافة المستجدات والعروض التي هي محل اهتمامك