Columbia Agar is used with or without blood for the isolation and cultivation of a wide variety of fastidious microorganisms. Conforms to Harmonized USP/EP/JP Requirements. Columbia Agar is not intended for use in the diagnosis of disease or other conditions in humans.
A medium recommended by the Harmonized USP/EP/JP for isolation and identification of Clostridia from non-sterile products. Conforms to Harmonized USP/EP/JP performance specification. Originally described as a general purpose nutritious agar base by Ellner et al. at Columbia University that can be enriched by the addition of sterile blood. The peptone mixture and yeast extract provide a source of nitrogen, essential vitamins and amino acids. The starch provides a carbon source and sodium chloride maintains osmotic balance. The Harmonized European Pharmacopoeia states that where necessary, gentamicin sulfate at a concentration of 20mg/L can be added after sterilization to reduce the growth of non-target organisms. According to the Harmonized European Pharmacopoeia, Reinforced Medium for Clostridia is used as a selective enrichment broth, with subculture performed onto Columbia Agar.
| Formula | Liter |
| Pancreatic Digest of Casein | 10.0 g/L |
| Meat Peptic Digest | 5.0 g/L |
| Heart Pancreatic Digest | 3.0 g/L |
| Yeast Extract | 5.0 g/L |
| Maize Starch | 1.0 g/L |
| Sodium Chloride | 5.0 g/L |
| Agar | 12.0 g/L |
Final pH: 7.3 ± 0.2 at 25 C
Formula may be adjusted and/or supplemented as required to meet performance specifications.
Preparation
- Dissolve 41 g of the medium in one liter of purified water.
- Heat with frequent agitation and boil for one minute to completely dissolve the medium.
- Autoclave at 121°C for 15 minutes.
- Cool 45-50°C.
Product was previously known as Acumedia Product: 7734 Columbia Agar; and LabM Product: HP012 Columbia Agar (USP/EP/JP)
Order Details
| Product # | Product Description |
| NCM0013A | Columbia Agar, 500 g |
| NCM0013B | Columbia Agar, 5 kg |
| NCM0013C | Columbia Agar, 10 kg |
| NCM0013D | Columbia Agar, 25 kg (European only size) |
| NCM0013E | Columbia Agar, 50 kg (US only size) |
Reference Material
NCM0013 - Columbia Agar, Safety Data Sheet [Danish] [Dutch] [English] [EU English] [Finnish] [French] [German] [Italian] [Spanish, Mexico] [Swedish]
NCM0013 - Columbia Agar, Technical Specifications Sheet [English]
For additional information or questions about product SDS documents, please contact Neogen at SDS@neogen.com.